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Mitochondrial membrane potential measurement by flow cytometry and airyscan microscopy Mitochondrial membrane potential (ΔΨm) was assessed in RA-differentiated SH-SY5Y cells subjected to chronic (9-day continuous exposure with media replacement every 2 days) or acute (8 h) treatments with FeSO₄ (50 or 100 µM) or BSO (25 or 50 µM), alongside untreated (UT) controls and a positive control for mitochondrial depolarization employing 20 µM CCCP (carbonyl cyanide 3-chlorophenylhydrazone
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