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Compatible empty columns Convenient prepacked columns Gluthatione Sepharose 4B resin is also available in the following formats: GSTrap 4B columns: HiTrap convenient format that can be used with a syringe, a peristaltic pump, or a chromatography system GST MultiTrap 4B: 96 well plate format for high throughput screening and small scale purification using a centrifuge or vacuum equipment GST GraviTrap: Gravity flow columns for direct purification of both clarified and unclarified cell lysates GST SpinTrap: Microcentrifuge based spin columns for rapid small-scale purification of GST-tagged proteins Other Cytiva products for recombinant protein purification Glutathione Sepharose 4B is optimized for high yield batch purification

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All flasks and tissue culture plates containing cells were incubated under standard culture conditions (37 °C in a water-saturated atmosphere of 5% CO 2 ) whilst pre-adipocyte growth media was refreshed every second day