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Right, volcano plot showing the relative fold change (log 2 ) in ER-metabolite abundance versus −log 10 (P value) from Slc33a1 -knockout KP1 cells expressing a vector control or SLC33A1 cDNA

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To understand the relevance of this phosphorylation we transfected HEK293T cells with different constructs of GABARAPL2, performed pulldowns of the mutants and used proteomics to analyze the variation in their interactomes (Fig