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Protein phosphorylation detection by mass spectrometry and site-directed mutagenesis reveal that T403, T404, T424, and/or T436 in the C-terminal domain of occludin are the phosphorylation sites targeted by PKCη and ζ
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Amino acids are sequentially added to resin, followed by cleavage, controlled oxidation to form the disulfide bond, and purification
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