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After determining the linear range of the reaction over time, pkat values of each protein in the presence of 1 mM γ-EC, 2.5 mM ATP and 100 mM glycine were measured in triplicate, and compared to a standard curve of free phosphate

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GHK-Cu is a small copper-binding tripeptide made of the amino acids glycine, L-histidine, and L-lysine (Gly-His-Lys) complexed with a copper(II) ion