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HEK293T cells were transfected with 1 µg lentiCRISPRv2 containing the sgRNA insert, 0.67 µg psPAX2 (Addgene, 12260) and 0.33 µg pVSVg (Addgene, 8454) using 6 µg polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)