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No specific labeling by NbLumSyt1, fused to a GFP-tag, was observed after incubating neurons from Syt1 knockout (KO) mice 18 , confirming specificity as also further confirmed by immunoprecipitation experiments, in which endogenous Syt1 was enriched from wildtype (WT) but not from Syt1-KO brain extracts ( Fig
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