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In the present study, we used a similar virtual screen procedure and the predicted allosteric site ( Figure 2A ) to screen for compounds with anti-inflammatory activity and novel chemical scaffold

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The method illustrated is based on the reaction of GSH with the thiol reagent DTNB (5,5-dithiobis(2-nitrobenzoic acid)) to form GSSG and TNB (5-thionitrobenzoic acid), which is detected spectrophotometrically at 412 nm ( Giustarini et al