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Fluorescence imaging MCF-7 cells were incubated with either plain PLGA nanoparticles (0.1 mg/mL) or PLGA nanoparticles labeled with AuNCs (0.1 mg/mL PLGA, 0.8 μg/mL AuNCs as gold) for 24 h in 10% FBS containing cell culture medium before imaging with confocal laser scanning microscope

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The use of ascorbate as a probe of opioid receptor structure: evidence for two independent mechanisms of receptor destruction by ascorbate