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National Institutes of Health Accessed October 29, 2025

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For RP separation, a Waters BEH Shield RP C 18 column (2.1 mm × 100 mm, 1.7 μm) was utilized, employing mobile phases consisting of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) for ESI + mode, and solvent A (5 mM ammonium acetate in water) with solvent B (5 mM ammonium acetate in acetonitrile) for ESI – mode