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To make pGEX-3X-TEAD1-YBD-C359A, pGEX-3X-TEAD2-YBD-C380A, pGEX-3X-TEAD3-YBD-C368A, pGEX-3X-TEAD4-YBD-C367A, the coding sequences for YBD of TEAD1-4 were PCR amplified from pCMX-GAL4-TEAD1 (Addgene #33108), pCMX-GAL4-TEAD2 (Addgene #33107), pCMX-GAL4-TEAD3 (Addgene #33106), pCMX-GAL4-TEAD4 (Addgene #33105), pRK5-Myc-TEAD4 (Addgene #24638), as two overlapping fragments with the mutations introduced in the primers for amplifying them, and cloned into the BamH1 site in PGEX-3X plasmid by NEBuilder® HiFi DNA Assembly
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Salazar R, Roepman P, Tabernero J, Schlicker A, Majewski I, Moreno V, Rosenberg R, Kopetz S , Wessels LFA, Bernards R, Simon I