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Cells were washed three times by gentle aspiration/addition of 2 mL aliquots of TAU uptake buffer (HEPES 20 mmol/L, NaCl 140 mmol/L, KCl 5.4 mmol/L, CaCl 2 1.8 mmol/L, MgSO 4 0.8 mmol/L and glucose 5 mmol/L) at 37 °C and equilibrated in 2 mL TAU uptake buffer at 37 °C

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However, no statistically significant differences in the number of nDNA lesions between the WT, the pbggcs - ko1 , or pbgr-ko mutant parasites were detected