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Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from the mouse kidney and HK-2 cells using a column-type animal tissue total RNA extraction and purification kit (Sangon Biotech Co., Ltd.), and then reverse transcribed to synthesize complementary DNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser Kit (Takara Bio, Inc.)

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Two of these proteins, GPX4 and ALOX15, which are associated with ferroptosis, were highlighted