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On the following day, rounded and floating spheres of NPCs were plated into matrigel-coated 6-wells and propagated until confluency

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Thus, absence of GPx1 in knockout mice was found to augment neuronal damage in the middle cerebral artery occlusion ischemia-reperfusion (MCAO) model of stroke, with increased infarct volume and increased apoptosis in brains of GPx1 −/− mice compared with wildtype mice [106]