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Western blot Cells were lysed in 1× RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min

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The α subunits have binding sites for IGF-1 and are linked by disulphide bonds (fig 3 ▶)

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