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Subsequently, ESCs were incubated overnight with rabbit primary antibodies against Vim (Proteintech, 10366-1-AP, 1:200, China) or FOSL2 (CST, 19967, 1:100, USA)

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Many supplement brands sell capsules in 250 mg or 300 mg strengths, often recommending 1–2 capsules per day (roughly 250–600 mg total)